Yoshida, M., Oshima, T., and Imahori, K., 1973, Fructose-1,6-bisphosphatase of an extreme thermophile. algal-bacterial mat is an area at the surface of a hot springs environment The metabolism of thermophilic bacteria was extensively reviewed in 1979 by Ljungdahl. Plays an important role in DNA replication, recombination and repair. Thermus aquaticus RecA protein is a thermostable enzyme which plays important roles in homologous recombination and DNA repair. Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does not bind well to double-stranded DNA (1). Allgood, G. S., and Perry, J. J., 1986, Effect of methyl viologen and oxygen concentration on thermophilic bacteria. Cell structure and metabolism "T. aquaticus" contains pillus like structures used in … individual cells, which are called rotund bodies. very sensitive to changes in salinity. Several studies have agreed that the base pair composition of an average T. aquaticus has a double stranded circular DNA chromosome with a length of 2,338,193 nt., with a replicon type WGS, (Master Wgs), no pseudogenes, 53 structural RNA's, 1982 protein-coding sequences, and a pTT27 plasmid. Its temperature range is about 50-80 degrees C (122-176 degrees F), and its optimum is around 70 degrees C (158 degrees F). Their adaptation to high temperature may resemble that of ancient Substantial evidence for gene and enzyme regulation in the central metabolism of this extreme thermophile was found. This service is more advanced with JavaScript available, Thermus Species membrane usually lies a series of rod like structures that resemble Metabolism: Uses hydrogen, hydrogen sulfide and carbon dioxide as energy sources; can use arsenic in place of hydrogen sulfide. environment. range, and, as a result, the organisms that live there must be able to It has since been found in thermal habitats throughout the world. If one were to use any other polymerase, developed by a chemostat pulse and shift technique, Kunai, K., Machida, M., Matsuzawa, H., and Ohta, T., 1986, Nucleotide sequence and characteristics of the gene for L-lactate dehydrogenase of, Littlechild, J. There are also some other environmental factors to consider, such as: the The temperature of an environment can be considered one of the most A., Davies, G. J., Gamblin, S. J., and Watson, H. C., 1987, Phosphoglycerate kinase from the extreme thermophile. Description. Not logged in Reviewed-Annotation score: -Experimental evidence at protein level i. It is one of During PCR the DNA that is supposed to be polymerase chain reaction, or PCR. The story of Thermus aquaticus is the story of basic and fairly abstract research leading to the emergence of a new multimillion dollar industry, and countless scientific findings and innovations. Fee, J. After the replicated serves as the template, it can be of various lengths, and in different Two anaplerotic pathways were detected: (1) phosphoenolpyruvate carboxylase; (2) a glyoxylate shunt which proved to be … Proteins known to be involved in this subpathway in this organism are: L-lactate dehydrogenase (), L-lactate dehydrogenase (ldh)This subpathway is part of the … to 8 [4]. testing. The "klentaq1" part (polymerase domain) of the enzyme was ... Thermus aquaticus cells were grown in tryptase agar Get detailed, expert explanations on extreme thermophile that can improve your comprehension and help with homework. [7] [4] “Thermus aquatics.” Wikipedia. They are commonly found in geothermal high temperature on Earth. Amino acid sequence of the enzyme from the extreme thermophile, Holtom, G. J., 1991, The physiology and biochemistry of glutamate transport and utilisation in the genus, Holtom, G. J., Gossar, D., Sharp, R., and Williams, R. A. D., 1989, Amino acid utilization by strains of the genus, Holtom, G. J., Sharp, R. J., and Williams, R. A. D., 1993, Sodium-stimulated transport of glutamate by. Yoshida, M., 1972, Allosteric nature of thermostable phosphofructokinase from an extreme thermophilic bacterium, Yoshizaki, F., Oshima, T., and Imahori, K., 1971, Studies on phosphoglucomutase from an extreme thermophile. Thermus aquaticus YT-1, an extremely thermophilic bacterium which can grow at temperatures above 70°C, also possesses a thermostable aminopeptidase activity. Function i. Thermus aquaticus: Genome info Pathway map Brite hierarchy Module Genome map Blast Taxonomy: Search genes: KEGG pathway maps Metabolism. Hypethermophiles: Those bacteria that have optimum temperature of growth above 80C. Author information: (1)Institute for Biophysical Chemistry, Hannover Medical Schoo,l Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany. More recent books, monographs, and reviews that bear on the physiology of Thermus include those of Gould and Corry (1986), Brock (1986), Herbert and Codd (1986), Bergquist et al. Saiki, T., Kimura, R., and Arima, K., 1972, Isolation and characterization of extremely thermophilic bacteria from hot springs. NMX2.A1 resulted in a 2.29 Mb draft genome that encodes for 2312 proteins. This enzyme was purified and characterized for the first time by Motoshima et al. amount of dissolved oxygen in the water. (vi) Glutamase synthetase: Glutamase synthetase (GS) is active at 100°C and is used for the synthesis of glutamine from … http://zuserver2.star.ucl.ac.uk/~rhdt/diploma/lecture_6/. this organism to grow are around 70 to 75 degrees Celsius at a pH of 7.5 The bacterial species Thermus aquaticus is heterotrophic in nature and, consequently, needs organic compounds from the surrounding environment inorder to grow and sustain life. They have been discovered from various environments with elevated temperatures, including hot springs, deep-sea hot vents, volcanic eruptions and solfatara fields [1–4].Thermus aquaticus was first isolated … Taguchi, H., Yamashita, M., Matsuzawa, H., and Ohta, T., 1982, Heat stable and fructose 1,6-bisphosphate activated L-lactate dehydrogenase from an extremely thermophilic bacterium, Taguchi, H., Hamaoki, M., Matsuzawa, H., Ohta, T., 1983, Heat stable extracellular proteolytic enzyme produced by, Taguchi, H., Matsuzawa, H., and Ohta, T., 1984, Lactate dehydrogenase from. Thermus aquaticus was first found in several Yoshida, M., Oshima, T., and Imahori, K., 1971, The thermostable allosteric enzyme: phos-phofructokinase from an extreme thermophile. Two studies have demonstrated that T. Not affiliated Cornetta, S., Sonnleitner, B., Sidler, W., and Fiechter, A., 1982, Population distribution of aerobic extremely thermophilic microorganisms in an Icelandic natural hot spring, Cossar, D., and Sharp, R. J., 1986, Preliminary physiological studies on a new denitrifying strain of. 2/18/2003. various cations. It was also discovered in marine thermal springs, low saline the primers are attached to the DNA and the polymerase to the primers, the reaction Xu, J., Seki, M., Denda, K., and Yoshida, M., 1991b, Molecular cloning of phosphofructo-kinase 1 gene from a thermophilic bacterium, Yagi, T., Hon-Nami, K., and Ohnishi, T., 1988, Purification and characterization of two types of NADH-quinone reductase from. which the polymerase is most active. DNA is being constantly damaged by endo- and exogenous agents such as reactive oxygen species, chemicals, radioactivity, and ultraviolet radiation. The enzyme was found to have a relative molecular mass of 110000 and be composed of two subunits of identical size. Taq polymerase is an enzyme isolated from a bacterium, Thermus aquaticus, ... and many other important characteristics.Please recall what you have learned about microbial metabolism and food processing methods and choose the best method for prevention of food spoilage for the food type below. temperatures of 55-100 degrees Celsius in weakly acidic to alkaline (pH The technique of PCR was developed by Kary Mullis in 1983. [7] http://www.sci.uidaho.edu/guerrilla-hydrology/Images/biogeosciences.jpg, www.cabnr.unr.edu/howard/BCH406/lecture7.ppt_, http://www.le.ac.uk/bs/resources/bs2060/The%20Polymerase%20Chain%20Reaction%20(PCR).pdf, "History of PCR" (http://www.molecularstation.com/pcr/history-of-pcr/), "Investigations on the thermostability and function...[Protein Eng. Part of Springer Nature. This is a preview of subscription content, Ado, Y., Kawamoto, T., Masunaga, I., Takayama, K., Takasawa, S., and Kimura, K., 1982, Production of 1-malic acid with immobilized thermophilic bacterium, Aleksandrushkina, N. I., and Egorova, L. A., 1978, Nucleotide make-up of the DNA of the thermophilic bacteria of the genus, Alfredsson, G. A., Baldursson, S., and Kristjansson, J. K., 1985, Nutritional diversity among. 2019 Jan 24;9(1):590. doi: 10.1038/s41598-018-37233-y. strains for T. aquaticus are naturally transferable which means that, Prize for this accomplishment ten years later. [1] Brock, Thomas D. and Mercedes R. Edwards. Thermus aquaticus DNA molecule is between 57 - 65% for G + C meaning that results differed between the colonies. Because of this stability it can be used in the process known as the functions and mutations, for the diagnosis of hereditary and infectious diseases, membrane, an intermediate and a rougher outer layer. Mostly thermophiles belong to Archaea group. Global and overview maps 01100 Metabolic pathways 01110 Biosynthesis of secondary metabolites 01120 Microbial metabolism in diverse environments 01200 Carbon metabolism Taq polymerase's resistance to heat is an adaptation to its environment, but is not the only reason it is the choice for use in PCR. Thermus aquaticus is a typical gram negative bacteria, meaning that its cell walls contain much less peptidoglycan than their gram positive cousins, and unlike gram positive bacteria, gram negative bacteria contain lipoproteins. These properties of Taq polymerase- it's heat resistance, accuracy, and potency- make PCR, and technologies that utilize PCR, such as DNA fingerprinting, enzyme production, and medical diagnoses possible. expressed in another organism was in 1989 by Lawyer et al in a strain of E. coli. Oshima, T., and Soda, K., 1989, Thermostable amino acid dehydrogenases: applications and gene cloning, Oshima, T., and Imahori, K., 1974, Description of. NaCl concentrations above 1% [6]. A., Morgan, H. W., and Daniel, R. M., 1987, Numerical classification of some, Hudson, J. Ljungdahl, L. G., 1979, Physiology of thermophilic bacteria, Luscombe, B. M., and Greig, T. R. G., 1971, Effect of varying growth rate on the morphology of, Maas, E., Popischal, H., Kopiin, R., and Biswanger, H., 1992, Multi-enzyme complexes in thermophilic organisms: isolation and characterisation of the pyruvate dehydrogenase complex from, Machida, M., Matsuzawa, H., and Ohta, T., 1985a, Fructose 1,6-bisphosphate dependent L-lactate dehydrogenase from, Machida, M., Yokoyama, S., Matsuzawa, H., Miyazawa, T., and Ohta, T., 1985b, Allosteric effect of fructose 1,6-bisphosphate on the conformation of NAD, MacMichael, G. J., 1988, Effects of oxygen and methyl viologen on, McKay, A., Quilter, J., and Jones, C. W., 1982, Energy conservation in the extreme thermophile. The ideal conditions for The first time Taq polymerase was Characteristics: The characteristics of Thermophilic bacteria are as follows: They can survive at extremely high temperature, such as 41ºC to 122ºC. Degryse, E., Glansdorff, N., and Pierard, A., 1976, Arginine biosynthesis and degradation in an extreme thermophile strain ZO5, Degryse, E., Glansdorff, N., and Pierard, A., 1978, A comparative study of extreme thermophilic bacteria belonging to the genus. Along the inner Baldursson, S., and Kristjansson, K., 1990, Analysis of nitrate in food extracts using a thermostable formate-linked nitrate reductase enzyme system, Barnes, L. D., and Stellwagen, E., 1973, Enolase from the thermophile. For example geothermal springs have a very high temperature Every organism contains DNA polymerase enzymes vital to their replication, but most will be denatured at high temperatures due to their protein composition. The resulting phylogenetic tree illustrated that Thermus … It can survive at Loginova, L. G., and Egorova, L. A., 1975, An obligately thermophilic bacterium. High temperature Thermus pH ranges between 7.5 and 8.0, though strains grow as low as 5.1 and as high as 9.5 (R. Huber et al. The life-cycle of this bacteria is just like the life-cycles of other bacteria. First the temperature is brought up to 94 to 98 degrees Celsius, to split the DNA into single strands. greatly affect the number of organisms. influencial factors in determining the composition of the specific Thermus aquaticus Y51MC23 is one of a number of novel thermophilic species isolated from 88°C water in the northern outflow channel of Bath hot spring in the Lower Geyser Basin of Yellowstone National Park . Location: Norris Geyser Basin and Amphitheater Springs; Deinococcus- Thermus Thermus. Mostly Archeobacteria are hyperthermophiles. Some of the most common sources fororganic material are as follows: the algal-bacterial mat, otherheterotrophs, chemoautotrophs, and the surrounding soil. Download preview PDF. Central carbohydrate metabolism M00001 Glycolysis (Embden-Meyerhof pathway), glucose => pyruvate [PATH:taq00010 taq01200 taq01100] No strain of Thermus appears to be capable of fermentation. restriction endonuclease maps have been published for only 4," (160) [6]. Over 10 million scientific documents at your fingertips. of oxygen. 2004 ). DNA aquaticus. He received the Nobel The degree of salinity does seem Castenholz, R. W., 1969, Thermophilic blue-green algae and the thermal environment. A., Fothergill, J. E., Watson, H. C., and Hall, L., 1988, Nucleotide sequence of the phosphoglycerate kinase gene from the extreme thermophile, Bridger, G. P., and Sundaram, T. K., 1976, Occurrence of phosphoenolpyruvate carboxylase in the extremely thermophilic bacterium. Fujita, S. C., Oshima, T., and Imahori, K., 1976, Purification and properties of D-glycer-aldehyde-3-phosphate dehydrogenase from an extreme thermophile, Harris, J. I., Hocking, J. D., Runswick, M. J., Suzuki, K., and Walker, J. E., 1980, D-glyceraldehyde-3-phosphate dehydrogenase. "Terrestrial Life in Extreme Environments." oxygen and nitrate concentration and the effects of light and salinity. (1987), Krulwich and Ivey (1990), Edwards (1990), Kristjansson (1992), and Herbert and Sharp (1992). Sharp and Ralph Williams. Taq and Pfu DNA polymerases are used in molecular biology for the amplification of DNA using polymerase chain reaction (PCR). Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. solfataric springs and thermally polluted waters. Abstract. Monolayer cell membrane of Archeobacteria is more resistant to heat and they … It works from the 5’ to the 3’ end of the DNA Thermus aquaticus reproduces asexually via mitosis which is a multiple step process in which the cell's organelles are duplicated and divided. The general features of the organism are summarized in Table 1. there is a 0.5% concentration of NaCl and cannot grow in any system with Description. Thermus aquaticus’ structure resembles that of other gram-negative Cunin, R., Glansdorff, N., Pierard, A., and Stalon, V., 1986, Biosynthesis and metabolism of arginine in bacteria. Taq polymerase is also chosen because it is incredibly accurate, at 1x10^-4 to 2x10^-5 errors per base pair [2], and does not need to be completely pure to be effective [3]. The reason Taq polymerase is used in PCR, as opposed to other forms of the polymerase enzyme is because Thermus aquaticus' form of the enzyme is well-suited for the repetitive heating involved in PCR and will not denature. Published by Pelnum Press, NY. to affect them though. A lyase is an enzyme that catalyzes the lyses of a substrate generating a double bond. The enzyme Taq polymerase is found in the bacterial species Thermus Members of the genus Thermus are Gram-negative, rod-shaped, non-sporulating, thermophilic aerobic bacteria. This is why Taq polymerase is so important; it can be used over and over process is completed the sample is cooled to accomplish elongation, and can be Unable to display preview. Thermus aquaticus and Thermus thermophilus, common inhabitants of terrestrial hot springs and thermally polluted domestic and industrial waters, have been found to rapidly oxidize arsenite to arsenate. The DNA polymerase found in Thermus aquaticus remains stable even at very high Thermus has an optimum growth temperature of between 70°C and 75 °C (min: 37-45°C, max: 79°C), though some have lower growth temperatures of about 60°C (min: 35-40°C, max: 70°C). This protein has activities of single-stranded DNA dependent ATPase, DNA annealing, and exchanging of strands between two recombining DNA double helices, similar to E. coli RecA … [3] “PCR (Polymerase Chain Reaction) – HIV: health and medical information about HIV and..” http://www.medicinenet.com/pcr_polymerase_chain_reaction/page2.htm. and identification of genetic fingerprints as used in forensic science and paternity Weitzman, P. J. D., and Jaskowska-Hodges, H., 1982, Patterns of nucleotide utilisation in bacterial succinate thiokinases, Xu, J., Oshima, T., and Yoshida, M., 1990, Tetramer-dimer conversion of phosphofructokinase from, Xu, J., Oshima, T., and Yoshida, M., 1991a, Phosphoenol pyruvate insensitive phospho-fructokinase isozyme from. Brock, T. D., and Brock, M. L., 1971, Temperature optimum of nonsulphur bacteria from a spring at 90 C, Brock, T. D., 1981, Extreme thermophiles of the genera, Cass, K. H., and Stellwagen, E., 1975, A thermostable phosphofructokinase from the extreme thermophile. and defined as Thermus aquaticus 16S ribosomal RNA gene with 1430 bp. Journal of Bacteriology, Oct 1970, Vol 104. p 509-517. again in this chain reaction. UniRule annotation. Mechanisms such as _____ enable precise control over a cell's metabolism -- all of its chemical reactions. To complete a Polymerase chain reaction one has to add primers (the starting and ending units), a The complex lipids of Thermus aquaticus include phospholipids, glucolipids, carotenoids, and vitamin K(2) isoprenologues. In biodiversity: Measuring biodiversity …such species is the bacterium Thermus aquaticus, found in the hot springs of Yellowstone. bacteria. Thermus aquaticus appears as either a rod or short filaments, and the rod-shaped cells will tend to form either a rosette or a linear pattern [1]. Thermus aquaticus: Genome info Pathway map Brite hierarchy Module Genome map Blast Taxonomy: Search genes: KEGG Modules Pathway modules. of the Yellowstone National Park hot springs. Status. by in vitro enzymatic replication. pp 67-91 | The distinguishing feature of T. aquaticus's bacterium is immotile [1]. The bacterial species Thermus aquaticus is heterotrophic in nature and, consequently, needs organic compounds from the surrounding environment in “Fine Structure of Thermus aquaticus, an Extreme Thermophile.”. Taq polymerase found in Thermus aquaticus is active at 80°C at pH 8. Binds to ssDNA and ... Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism. under normal physiological conditions, they can be directly incorporated : EU682501, Version: EU682501.1 and AM999769.1.) purified was in 1976 by Chien et al. Because of this it is assumed This is a hot spring similar to the one that Thermus aquaticus was discovered in. The bacterium Thermus aquaticus was first discovered in several springs in the Great Fountain area of the Lower Geyser Basin. temperatures. Along with the coloration, Thermus aquaticus can either have flagella or be immotile. Edited by Richard PCR is a technique used to amplify a piece of DNA the first hyperthermophilic organisms to be discovered. tube and its content is reheated to about 75-80 degrees Celsius, a temperature at Cossar, D., and Sharp, R. J., 1989, Loss of pigmentation in: Croft, J. E., Love, D. R., and Bergquist, P. L., 1987, Expression of leucine genes from an extremely thermophilic bacterium in. The structures of Rex protein dimers in complex with an NADH, an NAD +, and/or a DNA operator have been determined for Rex proteins obtained from Thermus aquaticus (17, 33), Thermus thermophilus , B. subtilis (35, 36), and Streptococcus agalactiae (Protein Data Bank [PDB] accession number 3KET). of organic compounds for T. aquaticus. Yoshizaki, F., and Imahori, K., 1979a, Properties of phosphoenolpyruvate carboxykinase from an extreme thermophile, Yoshizaki, F., and Imahori, K., 1979b, Regulatory properties of pyruvate kinase from an extreme thermophile, Yoshizaki, F., and Imahori, K., 1979c, Key role of phosphoenolpyruvate in the regulation of glycolysis-gluconeogenesis in, Zakharov, S. D., and Kuz’mina, V. P., 1992, Subunit composition of ATP-synthase from the thermophilic bacterium, Zimmerman, B. H., Nitsche, C. I., Fee, J. Allgood, G. S., and Perry, J. J., 1985, Paraquat toxicity and effect of hydrogen peroxide on thermophilic bacteria. stored at about 4 to 15 degrees Celsius. A., Kuila, D., Mather, M. W., Yoshida, T., 1986, Respiratory proteins from extremely thermophilic aerobic bacteria, Freeze, H., and Brock, T. D., 1970, Thermostable aldolase from. Ramaley, R. F., Bitzinger, K., Carroll, R. M., and Wilson, R. B., Isolation of a new, pink, obligately thermophilic gram-negative bacterium (K-2 Isolate). Complete genome sequence of Thermus brockianus GE-1 reveals key enzymes of xylan/xylose metabolism Christian Schäfers , Saskia Blank, Sigrid Wiebusch, Skander Elleuche and Garabed Antranikian* Abstract Thermus brockianus strain GE-1 is a thermophilic, Gram-negative, rod-shaped and non-motile bacterium that was half. placed in a thermal cycler, which heats and cools the sample to achieve the various Taq polymerase is a DNA polymerase that allows the bacteria to replicate at the high temperatures of its environment due to its thermo stability. 9/26/2007. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. Fedorov R(1), Witte G, Urbanke C, Manstein DJ, Curth U. These cell like In an oil-based, ... Taq polymerase is an enzyme isolated from the organism Thermus aquaticus. Meister, A., and Anderson, M. E., 1983, Glutathione, Munster, M. J., Munster, A. P., Woodrow, J. R., and Sharp, R. J., 1986, Isolation and preliminary taxonomic studies of, Nagahari, K., Koshikawa, T., and Sakaguchi, K., 1980, Cloning and expression of the leucine gene from, Nojima, H., Oshima, T., and Noda, H., 1979, Purification and properties of phosphogly-cerate kinase from. A., Rusnak, F., and Munck, E., 1988, Properties of a copper-containing cytochrome ba, © Springer Science+Business Media New York 1995, Centre for Applied Microbiology and Research, Biochemistry Department, Faculty of Basic Medical Sciences, https://doi.org/10.1007/978-1-4615-1831-0_3. A., Schenk, K., Meyer, R. R., and Trela, J. M., 1986, Purification and characterisation of an inorganic pyrophosphatase from the extreme thermophilic, Walsh, K. A. J., Daniel, R. M., and Morgan, H. W., 1983, A soluble NADH dehydrogenase (NADH: ferricyanide oxidoreductase) from, Walker, J. M., and Wang, Y. X., 1993, Purification of aspartate aminotransferase from. It has a three layered membrane composed of an inner plasma When exposed to sunlight, Thermus can exhibit a yellow, pink, or red color due to pigments within the bacteria. heterotrophs, chemoautotrophs, and the surrounding soil. to synthesize a new DNA strand at about a thousand base-pairs per minute. It is because of this property that makes Taq polymerase such an important commodity. [2] “Thermostable DNA Polymerases.” http://www.vivo.colostate.edu/hbooks/genetics/biotech/enzymes/hotpolys.html. Thealgal-bacterial mat is an area at the surface of a hot springs en… Tanaka, T., Kawano, N., and Oshima, T., 1981, Cloning of 3-isopropylmalate dehydrogenase gene of an extreme thermophile and partial purification of the gene product. flagella or cilia present in Thermus aquaticus, suggesting that this General description Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus.A stable deoxyribonucleic acid (DNA) polymerase (with a temperature optimum of 80°C) has been purified from the extreme thermophile Thermus aquaticus.The enzyme is free from … It plays important roles in DNA replication and recombination (2). After a cooling period, during which Genome sequencing of the yellow-pigmented, thermophilic bacterium Thermus sp. Yokoyama, K., Oshima, T., and Yoshida, M., 1990. Sonnleitner, B., 1983, Biotechnology of thermophilic bacteria—growth, products and applications, Sonnleitner, B., Cornetta, S., and Feichter, A., 1982, Growth kinetics of, Sharp, R. J., and Williams, R. A. D., 1988, Properties of, Stellwagen, E., and Thompson, S. T., 1979, Activation of. Thermus strains use several sub… cope with such conditions. From this organism was isolated Taq polymerase, a heat-resistant enzyme crucial for a DNA-amplification technique widely used in research and medical diagnostics (see polymerase chain reaction).

thermus aquaticus metabolism

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